Sensor and assay for screening of photosensitizers in living cells

Description:

Project ID:  D2018-33

 

Background

Photodynamic therapy (PDT) uses light and a photosensitizer in the presence of oxygen to elicit cell death. This technique has been under investigation for use in treatment of cancer

Most often used to treat skin cancer, mycosis fungoides, and non-small cell lung cancer.

Researchers need to detect the ability of PDTs to disrupt cell signaling in order to develop a cancer treatment. Currently, researchers look at measurement of single oxygen (1O2) or ROS induced oxidation. However, 1O2 and ROS can be the result of other cellular activities. Therefore, there is a need to directly quantify the efficacy of photosensitizers to examine the efficacy of PDT agents in living cells

 

Invention Description

Researchers at the University of Toledo have developed an assay that allows for the visualization of the action of photosensitizes in living cells. As a research tool, this technology allows for high-throughput screening of photosensitizers, including Rose Bengal, Methylene Blue, FMN, FAD, and Retinal. In the presence of light, singlet oxygen is created by photosensizers the cells, and producing reactive oxygen species, ultimately perturbing membrane bound molecules. This invention translates the perturbation of signaling molecules in cells to a fluorescence signal that can be easily detected using imaging plate reader.

 

Applications

Screening for efficacy of photosensitizers and visualization of membrane bound signaling molecule perturbation in living cells.

 

Advantages

•       Direct measure rather than measure of by-products

•       Detects influence photosensitizers on natural biomolecules in cells

•       Allows for measure of optimum chemical and light doses for PDT

 

Publications:        Ratnayake K, Payton JL, Lakmal OH, Karunarathne A.  Blue light excited retinal intercepts cellular signaling. Sci Rep. 2018 Jul 5;8(1):10207

 

 

Patent Information:
Category(s):
Research Tools
For Information, Contact:
Katherine Pollard
Licensing Associate
The University of Toledo
419-530-6228
katherine.pollard@utoledo.edu
Inventors:
Welivitiya Karunarathne
Kasun Ratnayake
Keywords:
PD2
Photodynamic therapy
Photosensitizer
PIP2