E. Coli plasmid (pET32) encoding MTA/SAH nucleosidase

Description:

Project ID: D2011-29

 

Gene: ‘pfs’ gene from H. pylori (strain J99) genomic DNA

 

Enzyme encoded: 5′-methylthioadenosine/S-adenosylhomocysteine nucleosidase

 

Plasmid: pET32

 

Description:

The bacterial enzyme 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) plays a central role in three essential metabolic pathways in bacteria: methionine salvage, purine salvage, and polyamine biosynthesis. Recently, its role in the pathway that leads to the production of autoinducer II, an important component in quorum-sensing, has garnered much interest. Because of this variety of roles, MTAN is an attractive target for developing new classes of inhibitors that influence bacterial virulence and biofilm formation. The pfs gene encoding MTAN was PCR amplified from Helicobactor pylori strain J99 and ligated into a derivative of E.Coli pET32.

 

References:

Donald R Ronning, Natalie M Iacopelli, and Vidhi Mishra. Enzyme–ligand interactions that drive active site rearrangements in the Helicobacter pylori 5′-methylthioadenosine/S-adenosylhomocysteine nucleosidase. Protein Sci. 2010 December; 19(12): 2498–2510.

Patent Information:
Category(s):
Research Tools
For Information, Contact:
Stephen Snider
AVP Tech Transfer
The University of Toledo
419 530 6225
Stephen.Snider@utoledo.edu
Inventors:
Donald Ronning
Keywords:
Autoinducer II
H. Pylori
MTAN
pET32
pfs gene