Magnetic Ionic Liquids Based Extraction and Purification of Nucleic Acids

Description:

Project ID # D2014-41

 

Background:

With a market of $3 billion in 2018 growing at a CAGR of 9.6 %, the nucleic acid isolation and extraction is one of the complex achievements in modern science. The analysis of deoxyribonucleic acid (DNA) plays a central role in a variety of applications such as determining microbial diversity in environmental samples, identifying pathogens in food, and monitoring the levels of cell free nucleic acids in cancer prognosis. Techniques such as polymerase chain reaction (PCR) and DNA sequencing enable the analysis of extremely small quantities of DNA, however often humic acids and proteins can inhibit purification and pre concentration of DNA. Conventional DNA purification methods of larger samples such as phenol-chloroform alkaline extraction, cesium chloride based density gradient ultracentrifugation, and solid phase extraction (SPE) involve time consuming, laborious centrifugation steps or an increased risk of contamination. Magnetic beads/particles are typically used as solid supports for the capture of DNA targets to improve sample throughput but aggregate over time resulting in lower capture efficiency and obstruction of liquid handling devices. Thus, a novel approach to purify and pre concentrate DNA for accurate and reproducible analysis is required.

 

Invention Description:

Researchers at the University of Toledo, have developed a novel method for the isolation and pre concentration of DNA from aqueous solution using Magnetic Ionic Liquid (MIL). The MIL-based method allows for rapid, highly efficient extractions providing a DNA-enriched micro droplet that is easily manipulated in aqueous solution by application of magnetic field. The novel particle-free approach is the first report of synthesis and characterization of ion-tagged oligonucleotides (ITOs) that partition into a hydrophobic MIL while retaining the ability to capture specific DNA sequences. When coupled with real-time quantitative PCR (qPCR), the MIL-supported ITO-based approach enables the detection of target DNA in aqueous samples containing genomic DNA interference with greater sensitivity than a typical biotin/streptavidin magnetic bead based method.

 

Applications:

Purification and isolation of Nucleic acid.

 

Advantages:

•       First patent issued for nucleic acid extraction and purification by magnetic ionic liquid.

•       High sensitivity and high recovery of target DNA compared to commercially available magnetic bead-based methods.

•       High capture efficiency and better at handling obstruction of liquid as compared to the current state of art.

•       Better detection and greater sensitivity over commercially available methods in targeting the DNA in aqueous samples when the novel MIL based method is coupled with qPCR.

•       The novel method possess unique physicochemical properties including hydrophobicity, low viscosity, and hydrolytic stability.

 

IP Status:    US Patent: US 10,280,416

 

Publications:

1.       Extraction of DNA by Magnetic Ionic Liquids: Tunable Solvents for Rapid and Selective DNA Analysis, ACS Publications

2.       Ion‐Tagged Oligonucleotides Coupled with a Magnetic Liquid Support for the Sequence‐Specific Capture of DNA, Angewalde Chemie International Edition

 

Patent Information:
Category(s):
Therapeutics
For Information, Contact:
Lokesh Mohan
Licensing Associate
The University of Toledo
419-530-6231
lokeshwar.mohan@utoledo.edu
Inventors:
Jared Anderson
Keywords:
DNA Extraction
DNA Purification
Magnetic Ionic Liquids (MILS)
Oligonucleotide