Description:
Project #: D1995-12e
Antigen Specificity: Beta – catenin
Clone: 5H10
Specificity: Reacts with ~92 kDa β-catenin protein
Host species: Mouse
Reactive species: Human, Mouse, Chicken
Description:
5H10 monoclonal antibody reacts with beta-catenin protein, a member of a catenin family. Catenins are intracellular proteins that interact with cadherins to mediate cellular adhesion. More specifically, beta-catenin binds to the cytoplasmic tail of E-cadherin. In addition, this molecule is a component of the canonical Wnt signaling pathway, a key player in such functions as embryonic development, the induction of cell polarity, and the determination of cell fate. In the absence of Wnt binding its receptor, beta-catenin is phosphorylated and resides in the cytoplasm where it is eventually targeted for degradation by ubiquitination. Upon Wnt binding, beta-catenin becomes dephosphorylated, translocates to the nucleus, and modulates gene expression in partnership with the transcription factors T cell factor (TCF) and lymphocyte enhancer binding factor (LEF). Expression of beta-catenin is found in a wide variety of non-immune and immune tissues, including thymocytes and T and B lymphocytes. The Wnt & beta-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and hematopoietic stem cells.
Applications: Western blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Flow cytometry
References:
- Wahl III, JK et al. The amino- and carboxyl-terminal tails of β-catenin reduce its affinity for desmoglein 2. J. Cell Sci. 2000; 113:1737–1745 [Link]
- Johnson, KR et al. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 1993; 207: 252–260 [Link]
- Abe, K et al. NMDA-receptor activation induces calpain-mediated β-catenin cleavages for triggering gene expression. Neuron 2007; 53(3): 387– 397 [Link]
- Wahl III, JK et al. N-cadherin-Catenin Complexes Form Prior to Cleavage of the Proregion and Transport to the Plasma Membrane. J. Biol. Chem. 2003, 278: 17269–17276 [Link]