E-Cadherin (4A2) Monoclonal Antibody

Project #:  D1995-23

Antigen Specificity: E-Cadherin (Cadherin 1)

Clone: 4A2

Specificity: Reacts with ~135 kDa E-Cadherin protein

Host species: Mouse

Reactive species: Human, Mouse, Rat

Description:

Epithelial cadherin (E-cadherin) or Cadherin-1, also known as CAM 120/80, is a protein that in humans is encoded by the CDH1 gene. CDH1 has also been designated as CD324. It is considered as an active suppressor of invasion and growth of many epithelial cancers.  E-Cadherin (4A2) Mouse mAb recognizes endogenous levels of total E-cadherin protein. This antibody does not cross-react with other cadherin proteins.

Applications: Western blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Flow cytometry

References:

1. Nieman, MT et al. Mechanism of extracellular domain-deleted dominant negative cadherins. J. Cell Sci. 1999; 112:1621–1632 [Link]
2. Johnson, KR et al. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 1993; 207: 252–60 [Link]
3. Rios-Doria, J et al. The role of calpain in the proteolytic cleavage of E-cadherin in prostate and mammary epithelial cells. J. Biol. Chem 2003; 278: 372–1379 n[Link]
4. Jaggi M. et al. E-cadherin phosphorylation by protein kinase D1/protein kinase Cμ is associated with altered cellular aggregation and motility in prostate cancer. Cancer Res. 2005, 65: 483–492 [Link]