Project ID: D1995-12d
Antigen Specificity: Beta – catenin
Clone: 6F9
Host species: Mouse
Specificity: Recognizes beta-catenin (94kD) in immunoblotting. Does not cross-react with plakoglobin
Reactive species: Human, Cow, Dog
Description:
The monoclonal antibody 6F9 reacts with human and mouse beta-catenin. Beta-catenin, along with alpha-catenin and gamma-catenin, belongs to the family of catenins. Catenins comprise a large family of Ca2+-dependent, homotypic cell-cell adhesion molecules that play important roles in development, epithelial cell polarity and tumor progression. Beta-catenin potentiates hyperproliferation and tumor formation. In the nucleus beta-catenin, it complexes with transcription factors and thus regulates the expression of specific genes. By its dual role, i.e. a structural role in cell-cell junctions and a regulatory role in the nucleus, beta-catenin can transduce changes in cell adhesion and junction formation to control transmembrane signalling and gene expression.
Applications: Western blot, Immunoprecipitation, Immunofluorescence
References:
1. Knudsen KA, Soler AP, Johnson KR, Wheelock MJ. Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin. J Cell Biol. 1995; 130(1): 67-77
2. Sacco, PA; McGranahan TM; Wheelock, MJ; Johnson, KR. Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin. J Biol Chem 1995, 270: 20201-6