Description:
Project #: D1995-16b
Antigen Specificity: Desmoglein 2
Clone: 6D8
Specificity: Reacts with ~165 KDa desmoglein-2 protein
Host species: Mouse
Reactive species: Human
Description:
The monoclonal antibody 6D8 recognizes the extracellular domain of human desmoglein 2. Desmoglein 2 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Human Desmoglein2 is a type I transmembrane glycoprotein of 1117 amino acid (aa) residues with a 23 aa signal peptide and a 25 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, desmogleins have R/YAL as the adhesion motif on its aminoterminal cadherin repeat. The cytoplasmic tails of desmogleins interact with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the desmogleins with the intermediate filaments. Desmoglein2 has been shown to be important in establishing cell cell adhesion and function in epithelial cells. Desmoglein2 was originally identified in colon carcinoma and colon, and was named HDGC (human desmoglein colon).
Applications: Western blot, Immunofluorescence, Immunoprecipitation
References:
1. Wahl, JK III, Nieset, JE; Sacco-Bubulya, PA; Sadler, TM; Johnson, KR; Wheelock, MJ. The amino- and carboxyl-terminal tails of (beta)-catenin reduce its affinity for desmoglein 2. J Cell Sci. (2000), 113 :1737-45
2. Arnemann, J; Spurr, NK; Magee, AI; Buxton RS. The human gene (DSG2) coding for HDGC, a second member of the desmoglein subfamily of the desmosomal cadherins, is, like DSG1 coding for desmoglein DGI, assigned to chromosome 18. Genomics (1992), 13(2), 484-6