Anti-Beta-Catenin (12F7) Monoclonal Antibody

Description:

Project #:  D1995-12c

 

Antigen Specificity: Beta – catenin

 

Clone: 12F7

 

Host species: Mouse

 

Reactive species: Human, Mouse, Rat, Pig

 

Description:

The 12F7 monoclonal antibody reacts with beta-catenin, one member of a family of catenins, which are intracellular proteins that interact with cadherins to mediate cellular adhesion. More specifically, beta-catenin binds to the cytoplasmic tail of E-cadherin. In addition, this molecule is a component of the canonical Wnt signaling pathway, a key player in such functions as embryonic development, the induction of cell polarity, and the determination of cell fate. In the absence of Wnt binding its receptor, beta-catenin is phosphorylated and resides in the cytoplasm where it is eventually targeted for degradation by ubiquitination. Upon Wnt binding, beta-catenin becomes dephosphorylated, translocates to the nucleus, and modulates gene expression in partnership with the transcription factors T cell factor (TCF) and lymphocyte enhancer binding factor (LEF). Expression of beta-catenin is found in a wide variety of non-immune and immune tissues, including thymocytes and T and B lymphocytes. The Wnt & beta-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and hematopoietic stem cells.

 

Applications: Western blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry - Parrafin

Patent Information:
Category(s):
Research Tools
For Information, Contact:
Stephen Snider
AVP Tech Transfer
The University of Toledo
419 530 6225
Stephen.Snider@utoledo.edu
Inventors:
James Wahl
Margaret Wheelock
Keith Johnson
Keywords:
Antibodies