Anti-Beta-Catenin (12F7) Monoclonal Antibody


Project #:  D1995-12c


Antigen Specificity: Beta – catenin


Clone: 12F7


Host species: Mouse


Reactive species: Human, Mouse, Rat, Pig



The 12F7 monoclonal antibody reacts with beta-catenin, one member of a family of catenins, which are intracellular proteins that interact with cadherins to mediate cellular adhesion. More specifically, beta-catenin binds to the cytoplasmic tail of E-cadherin. In addition, this molecule is a component of the canonical Wnt signaling pathway, a key player in such functions as embryonic development, the induction of cell polarity, and the determination of cell fate. In the absence of Wnt binding its receptor, beta-catenin is phosphorylated and resides in the cytoplasm where it is eventually targeted for degradation by ubiquitination. Upon Wnt binding, beta-catenin becomes dephosphorylated, translocates to the nucleus, and modulates gene expression in partnership with the transcription factors T cell factor (TCF) and lymphocyte enhancer binding factor (LEF). Expression of beta-catenin is found in a wide variety of non-immune and immune tissues, including thymocytes and T and B lymphocytes. The Wnt & beta-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and hematopoietic stem cells.


Applications: Western blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry - Parrafin

Patent Information:
For Information, Contact:
Stephen Snider
AVP Tech Transfer
The University of Toledo
419 530 6225
Margaret Wheelock
James Wahl
Keith Johnson