Description:
Project #: D1995-11
Antigen Specificity: Alpha – catenin
Clone: 1G5
Host species: Mouse
Reactive species: Human
Specificity: Recognizes the 102 kDa alpha-catenin protein
Description:
The monoclonal antibody 1G5 reacts with human alpha-catenin. Alpha-catenin belongs to the family of catenins. Catenins comprise a large family of Ca2+-dependent, homotypic cell-cell adhesion molecules that play important roles in development, epithelial cell polarity and tumor progression. α-catenin is a 102 kDa protein that is a key regulator of actin dynamics in cell-cell adhesion. During cell-cell adhesion, α-catenin forms a heterodimer with β-catenin and links the cadherins to actin associated with the cytoskeleton. Alpha-catenin also regulates the beta-catenin signaling in various cells. It displays the tumor suppressor activity and is found to be downregultaed in some forms of breast cancer.
Applications: Western blot, Immunoprecipitation, Immunofluorescence, Flow cytometry
References:
1. Johnson, KR; Lewis, JE; Li, D; Wahl, J; Soler, AP; Knudsen, KA; Wheelock, MJ. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 1993, 207: 252-60
2. Nieman, MT; Kim, JB; Johnson, KR; Wheelock, MJ. Mechanism of extracellular domain-deleted dominant negative cadherins. J Cell Science 1999, 112: 1621-32