Anti-Alpha-Catenin (1G5) Monoclonal Antibody


Project #:  D1995-11


Antigen Specificity: Alpha – catenin


Clone: 1G5


Host species: Mouse


Reactive species: Human


Specificity: Recognizes the 102 kDa alpha-catenin protein



The monoclonal antibody 1G5 reacts with human alpha-catenin. Alpha-catenin belongs to the family of catenins. Catenins comprise a large family of Ca2+-dependent, homotypic cell-cell adhesion molecules that play important roles in development, epithelial cell polarity and tumor progression. α-catenin is a 102 kDa protein that is a key regulator of actin dynamics in cell-cell adhesion. During cell-cell adhesion, α-catenin forms a heterodimer with β-catenin and links the cadherins to actin associated with the cytoskeleton. Alpha-catenin also regulates the beta-catenin signaling in various cells. It displays the tumor suppressor activity and is found to be downregultaed in some forms of breast cancer.  


Applications: Western blot, Immunoprecipitation, Immunofluorescence, Flow cytometry



1.      Johnson, KR; Lewis, JE; Li, D; Wahl, J; Soler, AP; Knudsen, KA; Wheelock, MJ. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 1993, 207: 252-60

2.   Nieman, MT; Kim, JB; Johnson, KR; Wheelock, MJ. Mechanism of extracellular domain-deleted dominant negative cadherins. J Cell Science 1999, 112: 1621-32

Patent Information:
For Information, Contact:
Stephen Snider
AVP Tech Transfer
The University of Toledo
419 530 6225
Margaret Wheelock
James Wahl
Keith Johnson