A431D Carcinoma Cell Line


Project ID:  D1995-22

A431D cells are a derivative of A431 cells that do not express a classical cadherin but do express α -actinin due to chronic treatment with dexamethasone. They also express catenins which are present in the cytosol. The A431D cell line is a derivative of A431 that has ceased to express cadherins (see Fig.)


Stable expression of most adherens junction components is depressed in A431D cells unlike A431 cells. A431D cells do not make detectable levels of E- or P-cadherin but upon immunoprecipitation, A431D cells synthesize close to normal levels of both α - and β-catenin.



Lewis, J. E., Wahl, J. K., Sass, K. M., Jensen, P. J., Johnson, K. R. and Wheelock, M. J. (1997). Cross-talk between adherens junctions and desmosomes depends on plakoglobin. J. Cell Biol. 36 (4), 919-934

Nieset JE, Redfield AR, Jin F, Knudsen KA, Johnson KR, Wheelock MJ. (1997) Characterization of the interactions of α-catenin with α-actinin and β-catenin/plakoglobin. J Cell Sci 110:1013–1022


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Kwaku Opoku
Licensing Associate
The University of Toledo
James Wahl
Margaret Wheelock
Keith Johnson