Mouse Glucocorticoid Receptor beta Polyclonal Antibody

Description:

Project ID: D2012-45

 

Antigen Specificity: Mouse GRβ

 

Clone: rMGRβ

 

Specificity: Detects mouse GRβ, but shows no reactivity to mouse GRα

 

Host species: Rabbit

 

Reactive species: Mouse

 

Description:

The polyclonal antibody recognizes the glucocorticoid receptor β (GRβ) in mouse. Glucocorticoid hormones control diverse physiological processes, including metabolism and immunity, by activating the major glucocorticoid receptor (GR) isoform, GRα. However, humans express an alternative isoform, human (h) GRβ, which can act as an inhibitor of hGRα to produce a state of glucocorticoid resistance, but which can also act independently of GRα to control a unique gene profile. In humans, evidence exists that hGRβ contributes to many diseases and resistance to glucocorticoid hormone therapy. Severe asthma, leukemia, ulcerative colitis, chronic sinusitis, systemic lupus erythematosus, and possibly cigarette smoking all correlate with overexpression of GRβ. To date, rigorous testing of the GRβ contribution to inflammatory and metabolic processes has not been possible, because rodents, especially mice, were not thought to express the β-isoform. Hinds et al demonstrated for first time, the expression of GRβ mRNA and protein in the mouse. It was also shown that mGRβ is highly expressed in muscle, liver, spleen and kidney and to a lesser extent in heart of mouse. In mice, a GRα-independent role for GRβ in control of lipid metabolism has recently been uncovered by Hinds et al (Unpublished). This antibody helps in the investigations on physiology and disease in much-needed rodent model of GRβ.

 

Applications: Western blot, Immunochemistry, Immunoprecipitation

 

References:

1.     Hinds, TDJ; Ramakrishnan, S; Cash, HA; Stechschulte, LA; Heinrich, G; Najjar, SM; Sanchez, ER. Discovery of glucocorticoid receptor-beta in mice with a role in metabolism. Mol Endocrinol 2010, 24, 1715-1727

2.     Oakley, RH; Cidlowski, JA. Cellular Processing of the Glucocorticoid Receptor Gene and Protein: New Mechanisms for Generating Tissue-specific Actions of Glucocorticoids. J Biol Chem 2011, 286,3177-84

3.     Bamberger, CM; Bamberger, AM; De Castro, M; Chrousos, GP. Glucocorticoid receptor beta, a potential endogenous inhibitor of glucocorticoid action in humans. J Clin Invest 1995, 2435-2441

4.     Oakley, RH; Sar, M; Cidlowski, JA. The human glucocorticoid receptor beta isoform. Expression, biochemical properties, and putative function. J Biol Chem 1996, 271, 9550-9559

5.     Kino, T., Manoli, I., Kelkar, S., Wang, Y., Su, Y. A. & Chrousos, G. P. Glucocorticoid receptor (GR) beta has intrinsic, GRalpha-independent transcriptional activity. Biochem Biophys Res Commun 381, 671-675 (2009)

Patent Information:
Category(s):
Research Tools
For Information, Contact:
Abhishek Sangal
Licensing Associate
The University of Toledo
419 530 6231
abhishek.sangal@utoledo.edu
Inventors:
Terry Hinds
Edwin Sanchez
Keywords:
Western blot
Immunochemistry
GRß
rMGRß
Glucocorticoid