Expression and Purification of Human P-12-LOX

Description:

Project ID: D2006-09

 

Enzyme: Human platelet 12-lipoxygenase (P-12-LOX)

 

Description:

Platelet 12-lipoxygenase (P-12-LOX) is overexpressed in different types of cancers, including prostate cancer, and the level of expression is correlated with the grade of this cancer. Arachidonic acid is metabolized by 12-LOX to 12(S)-hydroxyeicosatetraenoic acid [12(S)-HETE], and this biologically active metabolite is involved in prostate cancer progression by modulating cell proliferation in multiple cancer-related pathways inducing angiogenesis and metastasis. Thus, inhibition of P-12-LOX can reduce these two processes. Several lipoxygenase inhibitors are known, including plant and mammalian lipoxygenases, but only a few of them are known inhibitors of P-12-LOX.

 

Researchers at The University of Toledo expressed and purified the human P-12-LOX. This enzyme can be used to produce anti-human P-12-LOX antibodies and P-12-LOX inhibitors and is available for licensing purposes.

References:

1.        Jankun, J; Aleem, AM; Malgorzewicz, S; Szkudlarek, M; Zavodszky, MI; DeWitt, DL; Feig, M; Selman, SH; Skrzypczak-Jankun, E. Synthetic curcuminoids modulate the arachidonic acid metabolism of human platelet 12-lipoxygenase and reduce sprout formation of human endothelial cells. Mol Cancer Ther 2006, 5; 1371

2.        Amagata, T; Whitman, S; Johnson, TA; Stessman, CC; Loo, CP; Lobkovsky, E; Clardy, J; Crews, P; Holman, TR. Exploring sponge-derived terpenoids for their potency and selectivity against 12-human, 15-human, and 15-soybean lipoxygenases. J Nat Prod, 2003, 66: 230-235

3.        Basrur, V; Yang, F; Kushimoto, T; Higashimoto, Y; Yasumoto, K; Valencia, J; Muller, J; Vieira, WD; Watabe, H; Shabanowitz, J; Hearing, VJ; Hunt, DF; Appella, E. Proteomic analysis of early melanosomes: identification of novel melanosomal proteins. J Proteome Res, 2003, 2: 69-79

4.        Chen, XS; Brash, AR; Funk, CD. Purification and characterization of recombinant histidine-tagged human platlet 12-lipoxygenase expressed in baculovirus/insect cell system. Eur J Biochem, 1993, 214: 845-852

Patent Information:
Category(s):
Research Tools
For Information, Contact:
Stephen Snider
AVP Tech Transfer
The University of Toledo
419 530 6225
Stephen.Snider@utoledo.edu
Inventors:
Jerzy Jankun
Ansari Aleem
Ewa Skrzypczak-Jankun
Steven Selman
Keywords:
Cancer
Platelet 12-lipoxygenase (P-12-LOX)
Prostate